Isolation, Identification and Characterization of Bioflocculant-Producing Bacteria from Activated Sludge of Vulindlela Wastewater Treatment Plant

The low microbial flocculant yields and efficiencies limit their industrial applications. There is a need to identify bacteria with high bioflocculant production. aim of this study was isolate bioflocculant-producing bacterium from activated sludge wastewater characterise its activity. identification the isolated performed by 16S rRNA gene sequencing analysis. optimal medium composition (carbon nitrogen sources, cations inoculum size) culture conditions (temperature, pH, shaking speed time) were evaluated one-factor-at-a-time method. morphology, functional groups, crystallinity pyrolysis profile analysed using scanning electron microscope (SEM), Fourier transform infrared (FTIR) thermogravimetric (TGA) identified as Proteus mirabilis AB 932526.1. Its were: sucrose (20 g/L), yeast extract (1.2 MnCl2 (1 pH 6, 30 °C, inoculation volume (3%), (120 rpm) for 72 h cultivation. SEM micrograph revealed be amorphous. FTIR analysis indicated presence hydroxyl, carboxyl amino groups. completely pyrolyzed at temperatures above 800 °C. has potential produce importance.